Reovirus Low-Density Particles Package Cellular RNA

Properties of RNA transcriptase in reovirus subviral particles.

Subviral particles containing reovirus RNA transcriptase have been isolated from extracts of virus-infected mouse fibroblast cells. The purified particles which lacked the outer protein capsomeres of the mature virion had a buoyant density of 1.43-1.44 g/ml in CsCl and contained all of the double-stranded RNA genome of the intact virus. The particles were free of nuclease activity. RNA synthesi...

Characterization of low- and very-low-density hepatitis C virus RNA-containing particles.

The presence of hepatitis C virus (HCV) RNA-containing particles in the low-density fractions of plasma has been associated with high infectivity. However, the nature of circulating HCV particles and their association with immunoglobulins or lipoproteins as well as the characterization of cell entry have all been subject to conflicting reports. For a better analysis of HCV RNA-containing partic...

Pair density distribution function of membrane particles at low density.

Poliovirus Particles do not Form in Preinfected Cells with Reovirus

Background and Aims: Inhibition of viral growth in coinfected cells with two different viruses has been described. This phenomenon known as viral interference can occur in several virus host systems such as interference of enterovirus infection on poliovirus vaccine strains. In this study we superinfected reovirus infected HeLa cells with poliovirus to determine if poliovirus can replicate in s...

Transcription by infectious subviral particles of reovirus.

Digestion of purified reovirus with chymotrypsin in the presence of 0.15 M NaCl converts virions to infectious subviral particles (SVP(i)). The SVP(i) have an active ribonucleic acid (RNA) polymerase and are similar in composition to the partially uncoated virions which have been isolated from infected L cells. SVP(i) have a buoyant density of 1.40 g/ml in CsCl and sediment at 420S as compared ...